Description

Beckman Coulter OSR6127 Olympus LDH Reagent (Olympus Lactate Dehydogenase Test (LDH Test)) Specifications:

• Manufacturer: Beckman Coulter OSR6127
• Country of Origin: Ireland
• Application: Reagent
• Number of Tests: 4 X 640 Tests
• Storage Requirements: Requires Refrigeration
• Test Name: Lactate Dehydrogenase Test (LDH Test)
• Test Type: General Chemistry
• UNSPSC Code: 41116004
• Volume: R1 4x40ml, R2 2x20ml
• Beckman Coulter OSR6127 Olympus LDH Reagent Data Sheet
• Related Products
  • Olympus Lactate Reagent, Beckman Coulter OSR6193

Understanding Olympus LDH Reagent (Olympus Lactate Dehydogenase)

Serum Olympus Lactate dehydrogenase (LD) levels rise due to various conditions such as myocardial infarction, liver disease, anemias, pulmonary emboli, malignancies, and muscular dystrophy. Analyzing LD and CK (creatine kinase) isoenzymes together can confirm acute myocardial infarction.

Olympus LDH Reagent (Olympus Lactate Dehydogenase) Methodology

The LD test is based on the method developed by Wacker et al. It involves the conversion of Olympus lactate to pyruvate catalyzed by LD, producing NADH as a byproduct. NADH absorbs light strongly at 340 nm, allowing for measurement of LD activity in the sample based on the rate of change in absorbance at this wavelength.

System Information

For AU400/400e /480, AU600/640/640e /680 and AU2700/5400 Beckman Coulter Analyzers.

Reagents (LDH reagent)

Final Concentration of Reactive Ingredients: AMP Buffer, pH 8.9 (37°C) 230 mmol/L Lactate 70 mmol/L NAD+ 10 mmol/L Also contains preservatives. Precautions 1. For in vitro diagnostic use. 2. Do not ingest. Harmful if swallowed. 3. Contains sodium azide as a preservative which may react with lead joints in copper plumbing to form explosive compounds. Even though the reagent contains minute quantities of sodium azide, drains should be well flushed with water when discarding the reagent.

Preparation Of Reagents (Olympus Lactate / LDH reagent)

The Olympus Lactate LD reagent is ready for use. No preparation is required. Storage and Stability 1. The unopened reagents are stable until the expiration date printed on the label when stored at 2 – 8°C. 2. Opened reagents are stable for 30 days when stored in the refrigerated compartment of the analyzer. Indications of Deterioration Visible signs of microbial growth, turbidity or precipitate, or any change in reagent color may indicate degradation and warrant discontinuance of use.

What is a Lactate Dehydogenase Test (LDH test)?

A lactate dehydrogenase test (LDH test) usually measures the level of lactate dehydrogenase (LDH) in a sample of your blood. In certain cases, LDH test levels are measured in samples of other body fluids. This includes testing fluid from the spine (cerebrospinal fluid), the belly (peritoneal fluid), and the chest (pleural fluid).

LDH is also called lactic acid dehydrogenase. It is an enzyme. An enzyme is a protein that speeds up certain chemical reactions in your body. LDH helps your cells make energy. It is found in almost all the tissues in your body. The largest amounts of LDH are found in your muscles, liver, kidneys, and red blood cells.

If disease or injury damages tissues that have LDH, their cells release the enzyme into your bloodstream or other body fluids. It’s normal to have some LDH in your blood and body fluids. But if your LDH levels are high, it may be a sign of certain diseases or injuries.

An LDH test alone can’t show what is damaging your tissues or where the damage is located. So, an LDH test is usually done with other types of tests that can help diagnose the problem.

Other names: LD test, lactic dehydrogenase, lactic acid dehydrogenase