Beckman Coulter OSR60117 Olympus Calcium Reagent, 4 x 700 Tests

Description

Beckman Coulter OSR60117 Olympus Calcium Reagent (Coagulation Reagents, Calcium Test), 4 x 700 Tests Specifications:

• Manufacturer: Beckman Coulter OSR60117
• Country of Origin: Ireland
• Application: Coagulation Reagents
• Number of Tests: 4 X 700 tests
• Storage Requirements: Requires Refrigeration
• Test Method: Arsenazo III
• Test Name: Calcium Test
• Volume: R1 4 x 15 mL
• Test Type: General Chemistry
• UNSPSC Code: 41116004
• Beckman Coulter OSR60117 Olympus Calcium Reagent Data Sheet
• Calcium (Arsenazo) reagent OSR65117 can not be used on the AU400/AU480/5800.
• Related Products
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Product Details: Beckman Coulter OSR60117 Olympus Calcium Reagent (Coagulation Reagents, Calcium Test)

Measurement of calcium is used in the diagnosis and treatment of parathyroid disease, a variety of bone diseases, chronic renal disease and tetany (intermittent muscular contractions or spasms).

Although more than 99% of body calcium exists in bones and teeth, it is the calcium in the blood that is of most concern clinically. The bones serve as a reservoir to maintain relative constancy of serum calcium by releasing calcium when required to prevent hypocalcemia and trapping calcium to prevent excessively high levels of serum calcium.

The uptake and release of calcium from bone are under the control of the parathyroid hormone. The percentage of ingested calcium absorbed decreases as the dietary calcium content increases, so the amount absorbed can remain relatively constant. The slight increase in absorption that occurs on a high-calcium diet is reflected in increased renal excretion.

Serum calcium exists in three forms:

1) free calcium ion, Ca2+, 50%,

2) protein-bound calcium, 45% and

3) complexed calcium, mainly with citrate, 5%.

The ionized calcium is physiologically most significant but has proven difficult to assay directly. It may be estimated from total calcium given a knowledge of the protein content and pH of the blood which strongly affects the level of ionized calcium. Levels of calcium are roughly inversely proportional to phosphorus levels.

Calcium ions are important in the transmission of nerve impulses, as a cofactor in several enzyme reactions, in the maintenance of normal muscle contractility, and in the process of coagulation. A significant reduction in calcium ion concentration results in muscle tetany.

A higher than normal concentration of calcium ions produces lowered neuromuscular excitability and muscle weakness along with other more complex symptoms. In disease, calcium concentration may be either higher or lower than normal. Normal levels are highest in children and decline gradually throughout life. Variations in serum calcium may be due to disease of the parathyroid gland, bone disease, defective absorption of calcium from the intestine, kidney disease, multiple myeloma and various other abnormalities.

Methodology: Beckman Coulter OSR60117 Olympus Calcium Reagent (Coagulation Reagents, Calcium Test)

This Calcium procedure is based on calcium ions (Ca2+) reacting with Arsenazo III (2,2’-[1,8-Dihydroxy-3,6-disulphonaphthylene-2,7-bisazo]- bisbenzenear-sonic acid) to form an intense purple colored complex.2,3 Magnesium does not significantly interfere in calcium determination using Arsenazo III. In this method, the absorbance of the Ca-Arsenazo III complex is measured bichromatically at 660/700 nm. The resulting increase in absorbance of the reaction mixture is directly proportional to the calcium concentration in the sample.

What is a Calcium Test?

A calcium test measures the amount of calcium in your blood. If there is too much or too little calcium in the blood, it may be a sign of a wide range of medical conditions, such as bone disease, thyroid disease, parathyroid disorders, kidney disease, and other conditions.

Calcium is one of the most important minerals in your body. About 1% of the calcium in your body is in your blood. The rest is stored in your bones and teeth. Having the right amount of calcium in your blood is necessary for your nerves, muscles, and heart to work properly. It also helps blood vessels move blood throughout your body and helps release hormones that affect many body functions.

Other names: total calcium, ionized calcium

What is Calcium Test used for?

A blood calcium test is used to check your general health. It’s also used to help diagnose or monitor many types of medical conditions, including conditions that affect your bones, kidneys, digestive system, thyroid, and parathyroid glands.

There are two types of calcium blood tests that measure different forms of blood calcium:

• Total calcium test measures all the calcium in your blood. You have two types of blood calcium that are normally present in about equal amounts:
  • “Bound calcium” is attached to proteins in your blood.
  • “Free calcium” is not attached to proteins. It’s also called ionized calcium. This form of blood calcium is active in many body functions.

  Normally, your body tightly controls the balance of bound and ionized calcium, so a total calcium test gives a good estimate of how much ionized calcium you have.

  A total calcium test is the most common test for blood calcium. It’s often part of a basic metabolic panel (BMP) and a comprehensive metabolic panel (CMP), which are both routine screening tests.

• An ionized calcium test measures only the “free calcium” in your blood that isn’t attached to proteins. An ionized calcium test is more difficult to do, so it’s usually ordered if the results of a total calcium test aren’t normal. You may also have this test if you have a condition that affects your body’s ability to balance the amounts of ionized and bound calcium in your blood, or if you are seriously ill or having surgery.