Beckman Coulter OSR6137 Olympus Bicarbonate Reagent, 4 x 580 Tests

Description

Beckman Coulter OSR6137 Olympus Bicarbonate Reagent Specifications:

• Manufacturer: Beckman Coulter OSR6137
• Country of Origin: Ireland
• Application: Reagent
• For Use With: For Beckman Coulter AU Analyzers
• Number of Tests: 4 X 580 Tests
• Sample Type: Human Serum / Plasma Sample
• Storage Requirements: Requires Refrigeration
• Test Method: Enzymatic Method (Forrester)
• Test Name: Bicarbonate
• Volume: 4 X 25 mL
• Test Type: General Chemistry
• UNSPSC Code: 41116004

Intended use

Beckman Coulter OSR6137 Olympus Bicarbonate Reagent for the quantitative determination of Bicarbonate in human serum and plasma on Beckman Coulter AU analyzers.

About Beckman Coulter OSR6137 Olympus Bicarbonate Reagent

Bicarbonate measurements (HCO3) are used in the diagnosis and treatment of numerous potentially serious disorders associated with changes in body acid-base balance. The determination of bicarbonate (HCO3) is used in conjunction with other clinical and laboratory information for the evaluation of acid-base status. An elevation of the Bicarbonate (HCO3) level may be observed in compensated respiratory acidosis and metabolic alkalosis. Low Bicarbonate (HCO3) levels may be observed in compensated respiratory alkalosis and metabolic acidosis. Additional laboratory determinations will permit differentiation between metabolic and respiratory conditions.

Methodology: Beckman Coulter OSR6137 Olympus Bicarbonate Reagent

The bicarbonate reagent utilizes the enzymatic method developed by Forrester et al. In this procedure bicarbonate (HCO3) and phosphoenolpyruvate (PEP) are converted to oxaloacetate and phosphate in the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). Malate dehydrogenase (MD) catalyzes the reduction of oxaloacetate to

malate with the concomitant oxidation of reduced nicotinamide adenine dinucleotide (NADH). This oxidation of NADH results in a decrease in absorbance of the reaction mixture measured bichromatically at 380/410 nm proportional to the Bicarbonate content of the sample.