Beckman Coulter OSR6116 Olympus Cholesterol Reagent

Description

Beckman Coulter OSR6116 Olympus Cholesterol Reagent Specifications:

• Manufacturer: Beckman Coulter OSR6116
• Country of Origin: Ireland
• Application: Reagent
• Number of Tests: 4×910 Tests
• Storage Requirements: Requires Refrigeration
• Test Name: Cholesterol
• Test Type: Cardiac / Lipids / General Chemistry
• UNSPSC Code: 41116004

Intended Use

Beckman Coulter OSR6116 Olympus Cholesterol Reagent for the quantitative determination of Cholesterol concentrations in human serum on Beckman Coulter AU analyzers.
*Cholesterol reagent OSR6516 for use on the AU2700/5400/680 system only.

Understanding Beckman Coulter OSR6116 Olympus Cholesterol Reagent

Cholesterol test measurements play a crucial role in diagnosing and managing disorders linked to excessive cholesterol in the blood, alongside lipid and lipoprotein metabolism disorders. Total serum cholesterol analysis is particularly valuable for identifying hyperlipoproteinemia, atherosclerosis, hepatic, and thyroid diseases. Additionally, assessing total and HDL cholesterols, along with triglyceride determination, provides essential insights into predicting coronary heart disease.

Methodology: Beckman Coulter OSR6116 Olympus Cholesterol Reagent

Researchers Flegg and Richmond initiated total cholesterol assays in saponified serum extracts using “cholesterol dehydrogenase.” Previously, Hernandez and Chaikoff, as well as Hyun et al., isolated a cholesterol ester hydrolase effective in liberating free cholesterol from cholesterol esters. Eventually, in 1974, Allain et al. and Rieschlau et al. successfully merged the esterase and oxidase into a single enzymatic reagent for total cholesterol determination. The Cholesterol reagent complies with the National Cholesterol Education Program’s (NCEP) performance criteria for accuracy. Cholesterol esters in serum undergo hydrolysis by cholesterol esterase (CHE).

The resulting free cholesterol undergoes oxidation by cholesterol oxidase (CHO) to cholest-4-en-3-one, accompanied by the generation of hydrogen peroxide (H202). Subsequently, this hydrogen peroxide reacts with 4-aminoantipyrine and phenol in the presence of peroxidase, producing a chromophore. The resulting red quinoneimine dye can be measured spectrophotometrically at 540/600 nm to determine the increase in absorbance.