Beckman Coulter OSR6221 Olympus Glucose Reagent Kit

Description

Beckman Coulter OSR6221 Olympus Glucose Reagent Kit Specifications:

• Manufacturer: Beckman Coulter OSR6221
• Country of Origin: Ireland
• Application: Reagent
• Test Method: Hexokinase Method
• Number of Tests: 4 X 1300 Tests
• Test Name: Glucose Hexokinase
• Volume: 1,300 Tests
• Test Type: General Chemistry
• UNSPSC Code: 41116004

*Beckman Coulter OSR6221 Olympus Glucose Reagent Kit for use on the AU2700/5400 system only.

Intended Use

Beckman Coulter OSR6221 Olympus Glucose Kit reagent for the quantitative determination of Glucose in human serum, plasma, urine and cerebrospinal fluid on Beckman Coulter AU analyzers.

Understanding the Beckman Coulter OSR6221 Olympus Glucose Reagent Kit

The Glucose Kit is used to measure serum glucose levels, which can be either too high (hyperglycemia) or too low (hypoglycemia). These measurements are crucial in diagnosing and treating disorders related to carbohydrate metabolism, such as diabetes mellitus, neonatal hypoglycemia, idiopathic hypoglycemia, and pancreatic islet cell carcinoma.

In healthy pregnant women, the presence of glucose in urine (glucosuria) is common. However, the levels can vary significantly from day to day and throughout the day during pregnancy. It’s important to note that glucose isn’t normally found in urine from non-pregnant patients.

Measuring glucose levels in cerebrospinal fluid (CSF) helps distinguish between bacterial and viral meningitis. In bacterial and tuberculous meningitis, CSF glucose levels are typically low (less than 40% to 45% of simultaneous serum glucose levels), while they’re generally normal in viral meningitis. Carcinomatous meningitis, characterized by tumor cell infiltration of the meninges, also lowers CSF glucose levels.

Methodology: Beckman Coulter OSR6221 Olympus Glucose Reagent Kit

The Glucose Kit employs the hexokinase G-6-PDH method introduced by Stein. This method phosphorylates glucose using hexokinase (HK), adenosine triphosphate (ATP), and magnesium ions to produce glucose-6-phosphate (G-6-P) and adenosine diphosphate (ADP). Glucose-6-phosphate dehydrogenase (G6P-DH) then oxidizes G-6-P to 6-phosphogluconate, simultaneously reducing nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide, reduced (NADH). The change in absorbance at 340/380 nm corresponds to the amount of glucose present in the sample.