Beckman Coulter OSR61154 Olympus Ammonia Reagent R1 and Standard (AMMON)

Description

Beckman Coulter OSR61154 Olympus Ammonia Reagent R1 for Ammonia Test (Olympus AMMON) Specifications:

• Manufacturer: Beckman Coulter OSR61154
• Country of Origin: United States
• Application: Reagent (Olympus Ammonia Reagent R1)
• Container Type: Cartridge
• For Use With: For Olympus AU400, AU400e, AU600, AU640, AU640e, AU680, AU2700, AU5400 Chemistry Analyzers
• Number of Tests: 2 X 106 Tests
• Storage Requirements: Requires Refrigeration
• Test Name: Ammonia Test
• Test Type: General Chemistry
• UNSPSC Code: 41116004
• Volume : 2 X 16 mL, 1 X 3 mL
• Data Sheet
• Related products:
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Beckman Coulter OSR61154 Olympus Ammonia Reagent R1 for Ammonia Test (Olympus AMMON) : Intended Use

Reagent for the quantitative determination of Ammonia (NH3) concentrations in human plasma on the Beckman Coulter AU® chemistry analyzers.

Beckman Coulter OSR61154 Olympus Ammonia Reagent R1 for Ammonia Test (Olympus AMMON) : Summary

Ammonia, derived from the catabolism of amino acids and from the action of intestinal bacteria on dietary protein, is converted to urea in the liver hepatocytes and so rendered nontoxic. Under normal circumstances the concentration of ammonia in the circulation remains low, typically less than 50 µmol/L (85 µg/dL). Studies have shown that excess ammonia
can have a toxic effect on the central nervous system and clinical manifestations are typically neurological disturbances.

Elevated levels of ammonia may be either due to: (i) Inborn errors of metabolism; or (ii) Secondary to other conditions.
Inborn errors of metabolism are the major cause of elevated ammonia in infants and usually the result of urea cycle enzyme defi ciencies. Inherited disorders affecting the metabolism of the dibasic amino acids (lysine and ornithine) and those involving the metabolism of organic acids may also produce elevated levels of circulating ammonia.

Elevated ammonia may also be observed in severe liver failure as may occur in Reye’s Syndrome, viral hepatitis or cirrhosis.

Beckman Coulter OSR61154 Olympus Ammonia Reagent R1 for Ammonia Test (Olympus AMMON) : Methodology

A number of methods have been developed for the estimation of plasma ammonia and these can be broadly classified into either indirect or direct methods. In the indirect procedures, ammonia is first of all isolated, for example by the addition of alkali or the use of a cation exchange resin, after which it is measured colourimetrically by nesslerization or Berthelot
reaction. These procedures are not easily automated or require dedicated equipment. Direct procedures, such as enzymatic methods, are more widely used in routine laboratories as they do not require the separation of ammonia from the specimen prior to the analytical step. Direct procedures are therefore more easily automated. The Beckman Coulter OSR61154 ammonia reagent R1 is a direct enzymatic procedure based on the following reaction sequence:-

 

What is an ammonia levels test?

An ammonia test measures the amount of ammonia in a sample of your blood. Ammonia is also called NH3. It is a normal waste product in your body. Healthy bacteria in your intestines make ammonia when you digest protein in the foods you eat.

Normally, your liver changes ammonia into another waste product called urea. Your kidneys get rid of urea in urine (pee). This process is called the urea cycle.

When you’re healthy, the urea cycle prevents ammonia from building up in your blood. This is very important because ammonia is toxic (poisonous) to your brain. Even small increases in the level of ammonia in your blood can cause permanent brain damage, coma, and even death.

Liver disease is the most common cause of high ammonia levels. Other causes include kidney failure and genetic disorders called urea cycle disorders.

The medical term for high ammonia levels is hyperammonemia.

Other names: NH3 test, blood ammonia test, serum ammonia, ammonia; plasma

Olympus Ammonia Reagent R1 for Ammonia Test: What is it used for?

An ammonia test may be used to help diagnose high ammonia levels in people who have symptoms. The test alone can’t diagnose conditions that increase the amount of ammonia in your blood. But it may be used with other tests to help find the cause.

Ammonia levels testing can help diagnose conditions such as:

• Hepatic encephalopathy. This condition happens when toxins (poisons), including ammonia, build up in your brain because your liver is unable to break them down. It can cause memory loss, confusion, loss of consciousness, and coma.
• Reye syndrome. This rare disease damages the brain and liver. Without treatment, it causes death. It mostly happens in children younger than 15 who have had a viral infection that causes a fever, such as chickenpox or the flu. Taking aspirin during a viral illness may increase the risk of Reye syndrome.
• Urea cycle disorders (UCDs). This is a group of rare genetic conditions that you inherit from your parents. If you have a UCD, you lack enzymes that help change ammonia into urea. An enzyme is a protein that speeds up certain chemical reactions in your body. Symptoms may show up shortly after birth or later in life. Newborns are tested for certain UCDs as part of routine newborn screening. Your child’s health care provider can explain more about which UCD screening tests are used in your state.

Ammonia levels testing is also used to monitor conditions that cause high ammonia levels and to check if treatment is working.