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OSR6286 Olympus Iron Reagent, 4 x 970 Tests


Intended Use

Beckman Coulter OSR6286 Olympus Iron Reagent for the quantitative determination of Iron in human serum on Beckman Coulter AU analyzers.

Brand: Beckman Coulter
Manufacturer SKU: OSR6286
Analyzer Type: Chemistry Analyzer
Analyzer Series: AU Analyzer Series
Test Type: General Chemistry
Test Name: Iron
SKU: OSR6286 Category:


  • Manufacturer: Beckman Coulter OSR6286
  • Country of Origin: Ireland
  • Application: Reagent
  • Number of Tests: 4 X 970 Tests
  • Test Name: Iron
  • Test Type: General Chemistry
  • UNSPSC Code: 41116004
Understanding Iron Measurements

Iron measurements are essential in diagnosing and treating diseases like iron deficiency anemia, hemochromatosis, and chronic renal disease. Transferrin, a protein in the serum, carries most of the iron in the blood.


In 1954, Schade et al. introduced a method for directly determining serum iron levels. This involved incubating serum with a phosphate buffer, ascorbic acid, and terpyridine. In 1966, Goodwin proposed a similar method using an acetate buffer and bathophenanthroline, which improved accuracy by reducing random iron contamination and enhancing color development.

The Beckman Coulter method builds upon these techniques, utilizing TPTZ (2,4,6-Tri-(2-pyridyl)-5-triazine) as the chromogen. In an acidic environment, transferrin-bound iron releases as ferric ions, which are then reduced to the ferrous state using hydrochloric acid and sodium ascorbate. The ferrous ions react with TPTZ to form a blue complex, measurable at 600/800 nm. The increase in absorbance directly correlates with the amount of transferrin-bound iron.

OSR6286 Olympus Iron Reagent, 4 x 970 Tests
OSR6286 Olympus Iron Reagent, 4 x 970 Tests


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