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OSR6112 Olympus Total Bilirubin Reagent


Intended Use

Beckman Coulter OSR6112 Olympus Total Bilirubin Reagent for the quantitative determination of Total Bilirubin in human serum and plasma on Beckman Coulter AU analyzers.

*Total Bilirubin reagent OSR6512 for use on the AU2700/5400/680 system only.

Brand: Beckman Coulter
Manufacturer SKU: OSR6112
Analyzer Type: Chemistry Analyzer
Analyzer Series: AU Analyzer Series
Test Type: Hepatic
Test Name: Total Bilirubin
SKU: OSR6112 Category:


OSR6112 Olympus Total Bilirubin Reagent Specifications:

  • Manufacturer: Beckman Coulter OSR6112
  • Country of Origin: Ireland
  • Application: Reagent
  • Number of Tests: 4 X 590 Tests
  • Storage Requirements: Requires Refrigeration
  • Test Name: Total Bilirubin
  • Test Type: Hepatic / General Chemistry
  • UNSPSC Code: 41116004
  • Volume: 4 X 15 mL
About Olympus Total Bilirubin Reagent

Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder block.


Total bilirubin in serum is composed of direct (conjugated) bilirubin and indirect (unconjugated) bilirubin. Since the introduction of the diazo method for bilirubin determination by Ehrlich in 1883,1several modifications have been proposed to enhance the reaction.

The primary reason for these modifications is that while direct bilirubin couples directly with the diazotized compound to yield a colored product, the indirect portion of bilirubin requires a solubilizing agent, such as a surfactant. This Total Bilirubin Reagent is a variation of the classical method.

A stabilized diazonium salt, 3,5-dichlorophenyldiazonium tetrafluoroborate (DPD),reacts with bilirubin to form azobilirubin which absorbs at 570/660 nm. Caffeine and a surfactant are used as reaction accelerators. The absorbance at 570/660 nm is proportional to the bilirubin concentration in the sample. A separate serum blank is performed to eliminate endogenous serum interferences.

OSR6112 Olympus Total Bilirubin Reagent, 4 x 590 Tests
OSR6112 Olympus Total Bilirubin Reagent, 4 x 590 Tests


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