In Stock

OSR60117 Olympus Calcium Reagent, 4 x 700 Tests

$300.00

Intended Use

Beckman Coulter OSR60117 Olympus Calcium Reagent for the quantitative determination of calcium concentrations in human serum, plasma and urine on Beckman Coulter AU analyzers.

Brand: Beckman Coulter
Manufacturer SKU: OSR60117
Analyzer Type: Chemistry Analyzer
Analyzer Series: AU Analyzer Series
Test Type: General Chemistry
Test Name: Calcium
SKU: OSR60117 Category:

Description

OSR60117 Olympus Calcium Reagent, 4 x 700 Tests Specifications:

  • Manufacturer: Beckman Coulter OSR60117
  • Country of Origin: Ireland
  • Application: Reagent
  • Number of Tests: 4 X 700 tests
  • Storage Requirements: Requires Refrigeration
  • Test Method: Arsenazo III
  • Test Name: Calcium
  • Volume: R1 4 x 15 mL
  • Test Type: General Chemistry
  • UNSPSC Code: 41116004
  • Calcium (Arsenazo) reagent OSR65117 can not be used on the AU400/AU480/5800.
About Olympus Calcium Reagent

Measurement of calcium is used in the diagnosis and treatment of parathyroid disease, a variety of bone diseases, chronic renal disease and tetany (intermittent muscular contractions or spasms).

Although more than 99% of body calcium exists in bones and teeth, it is the calcium in the blood that is of most concern clinically. The bones serve as a reservoir to maintain relative constancy of serum calcium by releasing calcium when required to prevent hypocalcemia and trapping calcium to prevent excessively high levels of serum calcium.

The uptake and release of calcium from bone are under the control of the parathyroid hormone. The percentage of ingested calcium absorbed decreases as the dietary calcium content increases, so the amount absorbed can remain relatively constant. The slight increase in absorption that occurs on a high-calcium diet is reflected in increased renal excretion.

Serum calcium exists in three forms:

1) free calcium ion, Ca2+, 50%,

2) protein-bound calcium, 45% and

3) complexed calcium, mainly with citrate, 5%.

The ionized calcium is physiologically most significant but has proven difficult to assay directly. It may be estimated from total calcium given a knowledge of the protein content and pH of the blood which strongly affects the level of ionized calcium. Levels of calcium are roughly inversely proportional to phosphorus levels.

Calcium ions are important in the transmission of nerve impulses, as a cofactor in several enzyme reactions, in the maintenance of normal muscle contractility, and in the process of coagulation. A significant reduction in calcium ion concentration results in muscle tetany.

A higher than normal concentration of calcium ions produces lowered neuromuscular excitability and muscle weakness along with other more complex symptoms. In disease, calcium concentration may be either higher or lower than normal. Normal levels are highest in children and decline gradually throughout life. Variations in serum calcium may be due to disease of the parathyroid gland, bone disease, defective absorption of calcium from the intestine, kidney disease, multiple myeloma and various other abnormalities.

Methodology

This Calcium procedure is based on calcium ions (Ca2+) reacting with Arsenazo III (2,2’-[1,8-Dihydroxy-3,6-disulphonaphthylene-2,7-bisazo]- bisbenzenear-sonic acid) to form an intense purple colored complex.2,3 Magnesium does not significantly interfere in calcium determination using Arsenazo III. In this method, the absorbance of the Ca-Arsenazo III complex is measured bichromatically at 660/700 nm. The resulting increase in absorbance of the reaction mixture is directly proportional to the calcium concentration in the sample.

OSR60117 Olympus Calcium Reagent, 4 x 700 Tests
OSR60117 Olympus Calcium Reagent, 4 x 700 Tests

Reviews

There are no reviews yet.


Be the first to review “OSR60117 Olympus Calcium Reagent, 4 x 700 Tests”